Tiki Barber and the Giants represented the NFC in Super Bowl XXXV in 2001 but lost the game to the Baltimore Ravens. Established as a starter and playmaker, Barber was rewarded with a six-year contract from the Giants following the 2000 season. Following the signing, he called himself "the happiest man in New York right now."

Over the next four years Barber would become an integral part of the Giants' offense. In the last regular season game of 2002, Tiki exploded for 203 yards against the Philadelphia Eagles, at that time one of the league's best defenses. His performance helped the Giants clinch a playoff berth, but the team fell to the San Francisco 49ers in the wild-card round. He ended the season with 1,984 yards from scrimmage.

The 2003 NFL season saw the Giants finish an abysmal 4-12. During the 2004 NFL season, Barber reached the endzone for a career-high 15 touchdowns. He also rushed for 1,518 yards and notched 578 receiving yards for 2,096 total yards from scrimmage. On January 2, 2005, in the 2004 season finale at home versus the Dallas Cowboys, Tiki broke Rodney Hampton's Giants' all-time rushing record and Joe Morris's single-season rushing record in the same game. Poignantly, Barber broke the record on the final offensive play of the game, when he scored a game-winning touchdown to end an otherwise dismal 6-10 season for the Giants on a high point.

Barber retired from the NFL at the end of the 2006 NFL postseason as the Giants' all-time rushing and reception leader. Barber was inducted into the Virginia Sports Hall of Fame in 2011.

This New York Giants custom jersey was signed in black by Barber and includes a JSA sticker and COA.